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KMID : 0378019670100050057
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New Medical Journal
1967 Volume.10 No. 5 p.57 ~ p.63
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Effect of Panax Ginseng oand a Mineral-Vitamine-Antibiotics Complex on the Adrenal Ascorbic Acid Content of Rats Exposed to Continuous Cold or Heat Stress
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Abstract
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This study examined whether the Panax Ginseng and a mineral-vitamine antibiotics complex (called Nopcosol commercially) have any effects on the stress mechanism in rats exposed to continuous cold or heat.
Four hundred eighty male albino rats (body weight : 150200gm) were used. According to the kinds of food supplied (wheat, vegetable and fish, or wheat, vegetable and fish with added Nopcosol) and
1 drug administered (ginseng or saline), they were divided into wheat-saline group, wheat-ginseng group,
{ Nopcosol-saline group, and Nopcosol-ginseng group. Each group was further subdivided into control group, 1-day-exposure group, 5-day-exposure group, and 10-day-exposure group according to the duration of exposure to stress. Two experiments, one on heat stress and one on cold stress, were carried out.
For 6, 10 and 15 consecutive days the wheat-saline, wheat-ginseng, Nopcosol-saline, and Nopcosol-ginseng groups received each day 0.5ml per 100gm body weight of ginseng extract or physiological saline
r;t
r and ad libitum supply of wheat or wheat and Nopcosol (12 : 1). On the 5th day after beginning of ginseng or saline administration the animals undergoing stress were exposed to high (30C) or low (10C) temperature for 1, 5 or 10 days. The adrenal ascorbic acid content of all rats was measured
r immediately after termination of the last heat or cold stress.
Adrenal ascorbic acid content served as an indicator of reaction to stress. This vitamine was measured
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colorimetrically with Coleman Junior Spectrophotometer utilizing the reduction of 2, 6-dichlorophenol-indophenol sodium. To prepare ginseng extract, 300gm of Korean Panax Ginseng was extracted with 95% ethyl alcohol on a boiling water bath for about 300 hours. This procedure yielded 49 gm of dark brown substance from which a ginseng extract was prepared, dissolving 4mg of the substance in 1 ml "of physiological saline.
Results obtained were as follows:
F 1. Without exposure to cold or heat, the adrenal ascorbic acid content was not affected by the administration of ginseng extract.
2. The adrenal ascorbic acid content of rats fed on wheat, vegetable and fish with added Nopcosol was to ker~´ ihan´ that of rats fed on- wheat, vegetable, and "fish alone..
3. Under heat :tress, the adrenal ascorbic acid content of the wheat-saline group decreased progressive=
s-.;lyjeLween the first and the 5th day of exposure and then began to increase sluggishly, while under cold stress it increased somewhat ´between the first and the 5th day of exposure and then began to decrease until ´it showed a value significantly lower than that of pre-exposure period at the 10th day of exposure.
4. Under cold as well as heat exposure, the adrenal ascorbic acid content of the wheat-ginseng group decreased progressively between the first and the 5th day of exposure, but then began to increase until at the 10th day it almost regained the value before exposure.
5. Both in the warm and cold environments, the adrenal ascorbic acid contents of the Nopcosol-saline group and the Nopcosol-ginseng group did not differ significantly, and they varied similarly as the stress continued.
6. The adrenal ascorbic acid contents of rats fed on wheat with added Nopcosol decreased less or increased more prominently than that of the wheat-saline group between the first and the 5th day of exposure to heat or cold, respectively. However, the Nopcosol group and the wheat-saline group did_ not differ significantly from each other as to their adrenal ascorbic acid contents after the 5th day of exposure.
Assuming that increase in adrenal ascorbic acid content under chronic stress means growing resistance to stress, the above results may be interpreted as indicating that the ginseng contributes to the resistance against chronic heat and cold stresses. Under the same situation, Nopcosol may also help resisting the stress, but its effect does not last long. In addition, Nopcosol tends to nullify the effect of ginseng on the stress mechanism.
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